Stool collection kit MagSi

automated

Microbiome analysis involves studying the composition, diversity, and functional potential of microbial communities that inhabit various environments, including the human body, soil, water, and other ecosystems. The human microbiome, in particular, refers to the collection of microorganisms (bacteria, viruses, fungi, and more) that live on and inside the human body, influencing various aspects of health and disease. Microbiome analysis is conducted to understand the complex interactions between these microbes and their host, and how these interactions impact health, disease, and ecosystem functioning. Analyze your gut The MagSi-DNA Stool kit is intended for manual and automated isolation of microbiome DNA (mixture of bacterial/microbial and host DNA) from fresh, frozen or stabilized human stool samples. Processing time for the preparation of 96 samples is about 60 minutes including heat lysis incubations. The obtained DNA can be used directly for downstream applications such as PCR or NGS. Procedure MagSi-DNA Stool is optimized for use in isolating total DNA from human stool samples. The MagSi-ST10 magnetic beads are easy to handle and are supplied in a storage buffer for optimized resuspension and minimized sedimentation. Depending on the sample materials RNA may be co-purified. For efficient lysis of hard-to-lyse bacteria (e.g. gram positive bacteria) mechanical disruption with GP Lysis Tubes is recommended. If the microorganism of interest requires stronger homogenization than provided by a vortex, or if using a bead beater is desired, highpowered bead beating may be used, e.g. with the Geno/Grinder® (SPEX Sample Prep)