Static light scattering nanoparticle size analyzer Zetasizer Core

dynamic light scatteringlaserfor the pharmaceutical industry

Overview
The Zetasizer Core is a high-throughput light scattering platform that combines Dynamic Light Scattering (DLS), Static Light Scattering (SLS) and, in the DSF variant, Intrinsic Differential Scanning Fluorimetry (DSF) in a single microwell-plate workflow. It is designed to accelerate biologics discovery and development by delivering multi-parameter stability and structural data at scale. The system supports standard microwell plates (96, 384, 1536) and requires no proprietary consumables.

Key statistics

• Zero proprietary consumables (uses standard microwell plates)
• 4 µL minimum sample volume (1536-well plate)
• 5 clicks from setup to results (ZS Discover software)
• Up to 192 samples per minute throughput
• Built on 35+ years of DLS expertise

Features

• Combines DLS, SLS and DSF (DSF available in Zetasizer Core DSF) in a single automated plate workflow
• Dedicated SLS optical path for quantification of protein self-association and repulsion (A2/B22)
• Advanced thermal control for reproducible thermal ramps and structural stability data
• High-throughput screening across standard plate formats (96, 384, 1536)
• Automation-ready with robotic handling API support
• ZS Discover software: guided workflows, automatic multi-transition fitting, sample/solvent databases, acquisition-level review, 5-click workflow
• Implements Zetasizer Advance algorithms and adaptive correlation for trusted data quality

Key applications

• Proteins: candidate selection, developability assessment, aggregation and formulation screening, long-term stability studies
• Antibodies: candidate ranking, thermal stability comparison, aggregation monitoring, formulation optimization, comparability studies
• Lipid nanoparticles (LNPs): precision sizing, polydispersity and trace aggregate detection, batch-to-batch variability, QC and process development
• General industry uses: pharmaceutical development, academia, CRO/CDMO services for high-throughput stability profiling

Which model is right for you (summary)
Zetasizer Core DSF: Combines DLS and SLS with DSF to map sizing and aggregation across automated thermal ramps and capture structural unfolding events. Best for protein pre-formulation screening, structural biology R&D and workflows requiring thermal denaturation data (T_m).

Zetasizer Core: High-throughput DLS and SLS for physical and colloidal stability screening (sizing, aggregation, shelf-life). Best for routine formulation testing, QC and scale-up monitoring where thermal unfolding data is not required.

Additional notes
The platform supports barcode reader integration and plate presence sensing, modular field-replaceable units for reduced downtime, system self-diagnostics and remote support functionality. OmniTrust (21 CFR Part 11) compliance package and other options are noted as coming Q1 2027.

Caractéristiques / spécifications techniques

• Minimum sample volume (DLS, 1536-well): 4 µL
• Minimum sample concentration (example): 14 kDa, 0.125 mg/mL
• Particle size range (average dH): 0.6 nm – 2000 nm
• Minimum acquisition time: 1 sec
• Fitting methods: Malvern Panalytical Zetasizer cumulants and multimodal regularization; Auto-fit with multi-transition support for thermal data
• Advanced acquisition: Malvern Panalytical Zetasizer Adaptive Correlation
• SLS molar mass range:
• SLS resolution: 5% or 5 kDa, whichever is larger
• SLS sample concentration: 0.05 - 200 mg/mL (protein dependent)
• Fluorescence excitation: 280 nm; detection: 330 and 350 nm (sample volume 0.005 - 200 mg/mL protein dependent)
• Sample holders: 96, 384, 1536 plates (Ultra-low volume: 1536 = 4 µL; 384 round = 10 µL; 384 square = 40 µL; 96 = 100 µL)
• Instrument wavelength (light scattering): 830 nm
• Attenuation range: 1 to 10^5
• Temperature range: 4 - 95 °C; Temperature ramp rates: maximum 3 °C/min (thermal control for DSF workflows)
• Power: 100 - 240 V AC, 50-60 Hz, max. power 600 W
• Laser classification: Class 1
• Condensation control: Purge facility using dry air
• Sample throughput: measure up to 192 samples per minute (DSF/SLS)
• Thermal stability outputs: Tm, Tonset, Tagg (SLS), Tagg (DLS)
• Software & data management: multi-run workbooks, sample and solvent databases, acquisition-level review, 5-click workflow
• Hardware integrations: barcode reader, plate presence sensor, robotic handling API (option)
• Serviceability: modular field-replaceable units, system self-diagnostic, HW & SW error/use logging, Smart Manager remote support
• Dimensions: Width 620 mm, Depth 542 mm, Height 375 mm, Weight 29 kg