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  3. NGS reagent
  4. Menarini Silicon Biosystems

Quality control reagent kit DEPArray™ QC
NGSfor DNA analysisfor qPCR

quality control reagent kit
quality control reagent kit
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Characteristics

Applications
for quality control, NGS, for DNA analysis, for qPCR, FFPE tissues

Description

Formalin-fixation causes fragmentation and chemical modifications in DNA, posing significant challenges for molecular assays, such as downstream NGS applications. The DEPArray™ FFPE QC Kit has been developed to assess the DNA integrity of cell suspensions obtained from formalin-fixed paraffin-embedded (FFPE) samples of human origin prior to DEPArray™ experiments and NGS library preparation.DNA quality assessment is performed by a qPCR-based assay using two different primer pairs that produce amplicons of 54 bp and 132 bp. The quality of DNA is inferred by the ratio between the quantification using the long amplicon and the short amplicon (QC score). The QC score, coupled with ploidy information, allows to predict the outcome of NGS library preparation, providing a useful tool to infer the minimum recommended number of cells to obtain a given NGS performance level.

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