Rat/Mouse Insulin ELISA is suitable for measuring and quantifying insulin levels in metabolism and endocrine research. It has been used to determine plasma insulin by enzyme-linked immunosorbent assay. It has also been used to measure the insulin secretion from cells, in vitro.
Biochem/physiol Actions
Insulin is a key hormone that maintains normal blood glucose levels. It mediates cellular glucose uptake, controls carbohydrate, lipid, and protein metabolism, and enhances cell division and growth through its mitogenic effects. Insulin resistance is a condition of impaired sensitivity to insulin-mediated glucose disposal.
Disclaimer
For research use only. Not for use in diagnostic procedures.
General description
Insulin is secreted by the islet β cells in the pancreas. It contains two peptide chains linked by disulfide bridges. The A chain is composed of 21 amino acids and the B chain has 30 amino acids.
Other Notes
3 hour assay, room temperature 10 μL sample size
Research Category: Metabolism
Research Sub Category: Obesity/Metabolic Disorders
species reactivity
mouse, rat
packaging
kit of 1 × 96 wells
parameter
10 μL sample volume (4hr assay)
assay range
accuracy: 83-102%
linearity: 79-133%
standard curve range: 0.2-10 ng/mL
technique(s)
ELISA: suitable
input
sample type serum
sample type plasma (K2 EDTA)
UniProt accession no.
P01322
P01325
application(s)
research use
detection method
colorimetric (450nm/590nm)
shipped in
wet ice
storage temp.
2-8°C
Quality Level
200