CoolMPS High-throughput Sequencing Set is a novel antibody-based sequencing product. The dNTPs of CoolMPS are without fluorescent labeled (called cold dNTPs) which incorporated into the sequencing strand by DNA polymerase, and base calling is achieved by specific binding of fluorescently labeled antibodies. During this process, the incorporated bases are unmodified, ultimately resulting in clearer base calling.
Labeled antibodies lead to higher signal
Multiple labels per antibody, stronger signals
No quenching in CoolMPS
Natural base, no “scar” induced quenching
Polymerase provided natural base environment for binding and incorporation, reaction closer to completeness
Closer to 100% reaction>>Less signal decay>>Great potential for longer reads