Enzyme reagent kit forensicGEM

for DNA extractiontissuefor sperm analysis

Extracts DNA from sperm cells, semen samples, and vaginal swabs Produces single-stranded DNA validated for use with STR, PCR, and qPCR. Each extraction kit contains forensicGEM Sperm, Acrosolv, and ORANGE+ buffer MicroGEM’s forensicGEM™ Sperm lysis kit uses a novel cocktail of temperature-controlled enzymes to rapidly degrade sperm heads and digest the remaining tissue and nucleases in the sample. Our temperature-controlled DNA extraction process allows for lysis without purification and uses no reducing agents, such as DTT, that inhibit qPCR and STR analysis. As a result, DNA yields are maintained, processing time is quick, and opportunities for contamination are minimized making this the perfect approach for differential extractions and y-screening. Simplified, hands-off workflow provides STR, PCR, and qPCR-ready DNA in 15 minutes Sperm can be extracted in very small volumes and with very few sperm present because there is no loss of DNA to purification steps Reduced handling protects the integrity of the sample Single-tube processing is compatible with your existing thermal cyclers, making it easy to automate for quick DNA extractions. Sperm cells are a difficult type of cell to lyse, often requiring qPCR and STR-inhibiting chemicals such as SDS, mercaptoethanol and DTT, to lyse sperm cells. These chemicals are toxic to downstream processes and must be removed via purification steps. These steps are time-consuming and result in a loss of DNA. It is not possible to work with very small numbers of cells since DNA is lost at every step. The forensicGEM™ Sperm kit uses a unique mixture of thermophilic and mesophilic enzymes with no purification steps required.