Enzyme reagent kit RNAGEM
for RNA extractionfor RT-PCRRT-qPCR

enzyme reagent kit
enzyme reagent kit
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Characteristics

Type
enzyme
Applications
for RNA extraction, for RT-PCR, RT-qPCR

Description

This powerful, broad-specificity approach is ideal for gene expression from a very small number of cells, such as exosomes or single cells, without the need for harsh chemicals or further purification. Ideal for preparing RNA from mammalian cell culture, laser capture microdissection, FACS-prepared cell populations, cells in suspension, adherent cells, cells stored in RNAlaterTM and cell pellets Simplified, hands-off workflow provides RT-PCR and RT-qPCR ready RNA in approximately 5 minutes Releases both RNA and DNA with excellent recovery across a wide range of cell numbers Requires no further purification for accurate RT-PCR and RT-qPCR analysis Inhibitor-free means no harsh chemical washes or multiple steps RT-PCR and RT-qPCR reagents can be added directly to the lysate for a simple, streamlined protocol in 96 well plates Each kit contains RNAGEM, DNase I, BLUE buffer, 10x DNase buffer, and 10x TE RNAGEM is a whole nucleic acid extraction kit providing an extremely simple method to extract RNA from a single cell to 100,000 cells. The kit includes RNase-free enzymes, buffers, and DNase I. It enables temperature-controlled rapid preparation of RNA from mammalian cell culture, laser capture micro-dissections and FACS-prepared cell populations, such as macrophages. Simplified, hands-off workflow provides RT-PCR and RT-qPCR ready RNA in 5 minutes Releases both RNA and DNA with excellent linearity across a wide range of cell numbers Requires no further purification of the RNA for accurate RT-PCR and RT-qPCR analysis Inhibitor-free means no harsh chemical washes or multiple steps are required Reduced handling protects the integrity of the sample
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