NadPrep EZ DNA Library Preparation Kit v3 is designed for preparation of high-quality sequencing libraries from doublestranded DNA (dsDNA) on mainstream sequencing platforms. This kit optimizes the enzymatic fragmentation system to increase library yield while maintaining low background noise and significantly enhancing library complexity. This kit is suitable for multiple types of samples, including gDNA and FFPE DNA, and has been optimized for ultra-low-quality FFPE samples to ensure stable library yield. To simplify the experimental process, multiple processes were applied in one single step, including the fragmentation, end repair and A-tailing. This A-T ligation-based kit applies to the whole genome sequencing with DNA input ranging from 5 to 500 ng, and is compatible with hybridization capture based targeted sequencing.
Feature
Highly Suitable for various sample types, including gDNA and FFPE DNA of various grades (B+/B/C/D DNA)
Flexible system and simple operation, with fragmentation, end repair and A-addition being completed in one step
The length of DNA fragment is flexible and controllable, enhances enzymatic fragmentation recovery and library complexity.
Maintains ultra-low background noise (abnormal sequence introduced by enzymatic digestion by-products) even with poor-quality samples, reducing false positives and improving the accuracy of variant detection.
Flexible reaction system with excellent automation compatibility, seamlessly integrates with NGS workstations to reduce manual workload.