• Fast: 50 mins for 40 cycles
• Multiplex: Up to 5-plex probe in a reaction
• Antibody-coupled hot start Taq
• Probe type
• Enzyme Mix + 5X Reaction Buffer
• UDG system: Prevention of carryover contamination
qRT-PCR Kit [v6] (UDG System) is a quantitative RT-PCR reagent suitable for molecular diagnosis of SARS-CoV-2, which causes the new Coronavirus infectious disease (COVID-19). The kit's performance was confirmed using materials and methods approved by WHO on January 17, 2020. The enzyme mix is an optimized blend of Reverse Transcriptase, antibody-coupled hot-start Taq polymerase (Ab+Taq polymerase), RNase inhibitor protein, and a thermolabile UDG. These components help ensure sensitivity, accuracy, and specificity of PCR performance for COVID-19 molecular diagnosis. In addition, carryover contaminated PCR products can be removed by the applying the UDG system.
Application
• Quantification of target RNA by real-time RT-PCR
Character
• Uses the antibody-coupled Hot-Start Taq (Ab+Taq)
• Pre-denaturation time in PCR steps = < 5 min