Reverse transcriptase reagent RealHelix™ qRT-PCR Kit [v6]
RNase inhibitorfor RT-PCRRT-qPCR

reverse transcriptase reagent
reverse transcriptase reagent
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Characteristics

Type
reverse transcriptase, RNase inhibitor
Applications
for RT-PCR, RT-qPCR, for fast RT- qPCR
Tested parameter
protein
Micro-organism
SARS-COV-2, COVID-19, coronavirus
Other characteristics
hot start

Description

• Fast: 50 mins for 40 cycles • Multiplex: Up to 5-plex probe in a reaction • Antibody-coupled hot start Taq • Probe type • Enzyme Mix + 5X Reaction Buffer • UDG system: Prevention of carryover contamination qRT-PCR Kit [v6] (UDG System) is a quantitative RT-PCR reagent suitable for molecular diagnosis of SARS-CoV-2, which causes the new Coronavirus infectious disease (COVID-19). The kit's performance was confirmed using materials and methods approved by WHO on January 17, 2020. The enzyme mix is an optimized blend of Reverse Transcriptase, antibody-coupled hot-start Taq polymerase (Ab+Taq polymerase), RNase inhibitor protein, and a thermolabile UDG. These components help ensure sensitivity, accuracy, and specificity of PCR performance for COVID-19 molecular diagnosis. In addition, carryover contaminated PCR products can be removed by the applying the UDG system. Application • Quantification of target RNA by real-time RT-PCR Character • Uses the antibody-coupled Hot-Start Taq (Ab+Taq) • Pre-denaturation time in PCR steps = < 5 min

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