Sequentially isolate DNA and RNA in two separate eluates from the same FFPE sample. Xylene-free protocol
Mag-Bind® FFPE DNA/RNA 96 Kit is designed for the sequential isolation of DNA and RNA from the same formalin-fixed, paraffin-embedded (FFPE) tissue sample. The protocol utilizes non-toxic mineral oil in the place of the hazardous xylene for efficient deparaffinization of the FFPE sample. The specially formulated buffers reverse cross-linking without the need for overnight digestion resulting in high-yielding, high-quality nucleic acids. The isolation protocol allows for the extraction of both DNA and RNA in separate eluates from the same sample for a comprehensive analysis of both the nucleic acids. Purified DNA and RNA are suitable for a variety of downstream applications including SNP analysis, sequencing, and genotyping. The Mag-Bind® system is fully automatable on the Hamilton Microlab® STAR™, Tecan Fluent™, Thermo KingFisher® Flex™, and other open-ended workstations.
Extraction of both DNA and RNA in separate eluates from the same FFPE sample
No splitting of samples
Magnetic bead-based purification
For Research Use Only. Not for use in diagnostic procedures.
Downstream Applications NGS, PCR, qPCR, real-time RT-PCR, microarray, microRNA analysis
Elution Volume 50-200 µL
Starting Material FFPE tissue
Starting Amount < 3 FFPE sections of 10 μm thickness each
Throughput Up to 96
Processing Mode Automated, Manual
Technology Magnetic beads