Isolates RNA from small samples with lower elution volumes using spin columns
E.Z.N.A.® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as laser dissected samples (LDS) or fine needle aspirates (FNA). Normally, up to 5 x 105 eukaryotic cells or 5 mg tissue can be used in a single experiment depending on the type of tissue used. This kit allows processing of single or multiple of samples in less than 30 minutes. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10-15 µL nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern blotting, poly A+ purification, nuclease protection and in vitro translation.
For Research Use Only. Not for use in diagnostic procedures.
Downstream Application PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume 15-20 μL
Starting Material Cultured eukaryotic cells and soft tissues
Starting Amount Up to 5 x 105 eukaryotic cells or 5 mg tissue
RNA Yield Up to 50 µg
Processing Mode Manual, centrifugation
Throughput 1 - 24
RNA Binding Technology Silica Micro spin column
Binding Capacity 50 µg