Automatic sample preparation system Flongle

laboratorycellfor flow cytometry

Flongle™ has a disposable flow cell It is low cost It is small-scale It offers potential for pipette-free sample loading Interfaces with our current sequencing platforms For Research Use Only Note: This is largely a guide and is subject to change as Flongle evolves rapidly over this development period - please check this page regularly to ensure the instructions provided are correct. 1. Flongle: The flow cell dongle Introducing Flongle Flongle™ (Flow Cell Dongle) is a disposable flow cell with an adapter that provides pipette-free sample application; it is low cost and is a smaller-scale solution to real-time DNA sequencing than a MinION™Mk1B and Mk1C/GridION™/PromethION™ flow cell. cell lies on top (first tier), supported by the Flongle adapter (second tier), which itself interfaces with the MinION Mk1B (third tier) or GridION (not shown). It comprises a disposable flow cell containing the biological and chemical elements of nanopore sequencing. The reusable adapter contains a processor (Application Specific Integrated Circuitry (ASIC)) for processing the changes in current in each well, and provides an interface for use with a MinION Mk1B and/or GridION 2. The Flongle flow cell Loading a sample on a Flongle flow cell The Flongle has the potential of pipette-free sample loading of the prepared library onto the pore-embedded wells via a sample inlet port. The other port available to the user is a waste removal port; a third enables air venting and cannot be accessed. The sample loading port above the Nanopore array allows 30 µl of DNA library to be loaded into the sample reservoir (Fig. 2, green).