in-vitro microelectrode array system
The in vitro scratch assay is a straightforward and economical method of studying cell migration. By creating a gap on a confluent cell monolayer, the cells on the edge of the gap will move to close the “scratch”. However, existing methodologies lack consistency: handheld tools can disturb the cell monolayers if not used correctly. And for HTS experiments where thousands of well plates must be scratched consistently the preparation of assays can create a real bottleneck.
The HTSScratcher was designed in close cooperation with HTS researchers in order to remove research delays in the assay preparation phase. The apparatus provides support and guidance for a commercially available, commonly used handheld tool. The HTSScratcher’s four guidance shafts guarantee a perfect horizontal position of the scratch needle plate relative to the well plate beneath. When lowering the tool, the controlled counterforce mechanism ensures smooth, controlled downward movement.
When touching the well plate, the needle-tool can be moved horizontally over a determined distance, thus creating a scratch in the cell culture without damaging the plastic well plate. The sledge support which runs over eight polyamide rollers ensures smooth transport and rapid removal and loading of the well plates.