Analysis software MicroSight®treatmentevaluationlaboratory

MicroSight® MSI is a simple and fast method for evaluating microsatellite instability (MSI) status of human DNA. The analysis is based on PentaBase’s proprietary INA® technology that enables fast and easy detection of microsatellite instability status by real-time PCR and high-resolution melt (HRM) analysis. MicroSight® MSI requires very little hands-on time and enables evaluation of patient microsatellite status of purified human genomic DNA in less than one and a half hours for fast treatment guidance of cancer patients. MicroSight® MSI workflow. Only three fast and simple steps are needed for the analysis of microsatellite instability with MicroSight® MSI. The method is based on high-resolution melt analysis and gives a clear and objective evaluation of microsatellite status. Evaluation of well-established MSI markers in paired and unpaired tumour samples MicroSight® MSI PentaBase Panel evaluates instability of the well-established MSI markers BAT25, BAT26, NR22, NR24 and MONO27 allowing for easy conversion from current methods based on capillary electrophoresis. MicroSight® MSI can be used for analysis of both paired and unpaired tumour samples. Evaluation of unpaired tumour samples is done by comparison to a universal reference assay supplied by PentaBase (Strip HR). Up to three unpaired tumour samples can be analysed in each real-time PCR run.