Automated isolation of cell-free tumor or fetal DNA
from 3 - 5 ml plasma
In the event of apoptosis, necrosis or break-down of pathogens, or cells, such as white blood cells cfDNA is floating through the blood. As the average size of cfDNA is 170 bp (50 – 200 bp) and only 1 – 50 ng of cfNA/ml of plasma is present, it is quite difficult to separate this hay in the haystack. Nevertheless the detection of cfDNA in the blood is an elegant non-invasive method to detect or monitor tumors or predict chromosomal disorders without surgery. PerkinElmer chemagen has a new method to isolate cfDNA form 3-5 ml plasma within 80 minutes with the chemagic 360 instrument.
The kit is designed for the use with human plasma samples derived from EDTA, citrate or Streck Cellfree DNA BCT® tubes.
Fresh and frozen plasma can be used.
With the chemagic cfDNA 5k Kit H24 circulating cell-free DNA (cfDNA) can be isolated from 3 - 5 mL of plasma obtained from human whole blood samples. All the reagents needed for the isolation of cfDNA are included in the kit, with the exception of nuclease-free water for dissolving Proteinase K. The kit components (reagents and plastic ware) provide sufficient material for 240 preparations. The kit is designed to be used with the chemagic 360 and integrated chemagic Dispenser. The product is intended for professional users such as technicians and physicians trained in molecular biology techniques.
The chemagic cfDNA 5k Kit H24 is based on chemagic Technology using M-PVA Magnetic Beads for the isolation of cfDNA. The cfDNA binds to paramagnetic beads which are magnetically separated from the sample material.