CO2 sensor SP-CD1

laboratory

The CO2 sensor spots measure the partial pressure of dissolved carbon dioxide. The spots are fixed on the inner surface of glass or transparent plastic vessels. Measurements are then taken contactless from the outside through the transparent container material. Re-usable & disposable sensors Online monitoring Non-invasive & non-destructive measurement Measurement range from 10 - 250 hPa pCO2 (8 ... 180 mmHg) No consumption of carbon dioxide Measures carbon dioxide in liquids Beta-irradiated and autoclavable sensors available APPLICATIONS during mammalian cell culture (e. g. CHO cell-line). Biotechnology: Carbon Dioxide Monitoring in Process Development In bioprocess development & production, monitoring dissolved CO2 in addition to pH and O2 provides valuable information. It ensures stress-free cultivation conditions during mammalian cell culture (e.g. CHO cell-line). Constant pH and / or oxygen supply and sufficient nutrition levels are key parameters to optimize yield. Thereby, excess CO2 can act as a stressor or even a toxin to the culture and has to be controlled. The chemical optical CO2 sensors can enhance performance of process monitoring during cultivation in disposables, leading to process optimization. PreSens CO2 Sensors as an Alternative to Off-line Sampling PreSens CO2 sensors were evaluated for monitoring the cultivation cells in a 3-L bioreactor. The sensors show excellent long-term stability over a period of 10 days during cell cultivation of CHO cells with an initial seed density of 400,000 cells/mL. The readings of the PreSens CO2 sensor offer the possibility to continuously monitor the CO2 concentration between two off-line measurements.