Solution reagent AccuStart

NGSfor Sanger sequencingfor nucleic acids

Superior sensitivity and multiplexing for DNA amplification of long targets Features & Benefits Amplify +24 kb gDNA and +40 kb lambda DNA 4x concentration enables superior sensitivity with low inputs (100 pg) Multiplex up to 6 targets with each target up to 6 kb in length Stabilized, single-tube SuperMix minimizes pipetting errors and hands-on-time Reliable lot-to-lot reproducibility ensures consistent results Amplification through uracils and primers containing inosines/uracils Description The AccuStart Long Range SuperMix is a 4x, ready-to-use solution that contains all the components for long range target amplifications, including a blend of two hot-start thermostable DNA polymerases (one with strong proof-reading activity) and an optimized buffer to ensure high efficiency, sensitivity and specificity. The SuperMix enables routine and easy amplification of up to 24kb target from gDNA with high accuracy (> 10-fold better fidelity than Taq) and accommodates targets with broad GC-content (no separate GC buffer needed). This product is also capable of amplifying multiple targets simultaneously and is suitable for end point PCR, template prep for sanger sequencing, NGS, cloning and HLA typing. Superior Sensitivity: Improved yields across a range of DNA inputs and target sizes Higher yield than major competitors Inputs as low as 100 pg Strong yield at 24 kb length Clean and visible PCR bands Maximized Multiplexing: Amplify 6 targets up to 6 kb each The AccuStart Long Range SuperMix can multiplex 6 targets up to 6 kb each, further speeding up experiments, reducing costs and allowing for more data to be derived per run.