Seamless assembly of multiple DNA fragments for high efficiency cloning
Features & Benefits
Formulated to increase number of transformants
Assemble up to 6 fragment inserts without the need for restriction enzymes
Flexibility in design with 10x MM enabling assembly of low concentration DNA samples
Eliminates dilution step resulting in easier workflow and 1 hr assembly
Includes DpnI to reduce background when using plasmid templates for PCR
epliQa HiFi Assembly Mix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
The repliQa™ HiFi Assembly Mix simplifies the construction of recombinant DNA through seamless assembly of multiple DNA fragments in a single, isothermal reaction. Similar in principle to the Gibson Assembly® Method, the high efficiency repliQa HiFi Assembly Mix is ideal for a range of genetic engineering applications including:
Routine molecular cloning
Construction of libraries for directed evolution studies
The concentrated (10x), two component format allows flexibility in design of assembly reactions and compatibility with less concentrated DNA samples. The repliQa Mix has been optimized for use with a total input quantity of DNA fragments in the range of 0.03 to 0.5 pmols. Assembly of up to five DNA fragments is recommended, though the repliQa Mix has been used successfully for more complex assemblies. The mix supports assembly of multiple DNA fragments in a single 1-hr reaction.