Tough-tested real-time PCR
Features & Benefits
ToughMix additives neutralize PCR inhibitors to ensure reliable assay performance with a spectrum of starting materials including: clinical specimens, plants, soil, environmental or complex food matrices
Superior assay sensitivity and target precision with AccuStart™ II enzyme technology – maximum-yielding, engineered Taq DNA polymerase with stringent, ultrapure antibody hotstart
Easy-to-use 2X concentrated master mixes with AccuVue™ plate loading dye and optimized passive reference dye for simplified reaction setup
Supports efficient vortex mixing with proprietary anti-foaming technology
Consistent and reliable performance with exceptional reagent stability (uninhibited after 20X freeze-thaw or 30 days at 22°C)
PerfeCTa qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
PerfeCTa qPCR ToughMix is a 2X concentrated ready-to-use reaction cocktail for PCR amplification of DNA templates that relieves several types of PCR inhibition commonly encountered with crude extracts, environmental specimens, plant tissues, animal tissues, and complex food matrices. This robust real-time qPCR reagent provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. The only user-supplied components are primers, probe(s), and DNA template. Pre-blended with inert AccuVue plate loading dye to help minimize pipette errors during setup and provides visual confirmation of thorough mixing.