Advanced 1-tube SuperMix optimized to support highly multiplexed DNA amplifications in miniaturized reaction volumes and withstand a broad spectrum of PCR inhibitors
Features & Benefits
1-tube SuperMix minimizes pipetting steps, simplifying reaction assembly and improving accuracy
5x concentrated reagent provides more flexibility with dilute DNA samples sensitivity
ToughMix reagent technology neutralizes a broad spectrum of PCR inhibitors common in plant and animal tissues, environmental sources, clinical specimens and complex food matrices
Superior assay sensitivity and specificity with ultrapure AccuStart II enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hotstart
Supports efficient vortex mixing with proprietary anti-foaming additives
PerfeCTa Multiplex qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Suppression of low copy amplicons by high copy reference targets during multiplex co-amplification skews the apparent representation and quantification of low copy target sequences. PerfeCTa MultiPlex qPCR ToughMix transcends these limitations by enabling sensitive, broad linear dynamic detection range with co-amplification of four abundant (106) targets. PerfeCTa MultiPlex qPCR ToughMix enables multiplex qPCR assay performance comparable to single-plex qPCR probe assays without the need to rigorously titrate primer concentration.