Simplifies organic extraction of nucleic acid template and improves safety
Features & Benefits
Eliminates interphase contamination of nucleic acid solution
30% greater yield of nucleic acids over conventional method
Gel barrier allows easy sample decanting
Reduced contact with hazardous organic solvents
5PRIME Phase Lock Gel is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
5PRIME Phase Lock Gel (PLG) is a unique product that eliminates interphase-protein contamination during phenol extraction and ensures faster results with improved recoveries. PLG migrates to form a tight seal between the phases of an aqueous/organic extraction during centrifugation. The organic phase and the interphase materials are effectively trapped in or below the barrier, thus enabling complete and easy decanting or pipetting of the entire aqueous phase. The benefits are increased yields of up to 30%, increased protection from exposure to hazardous compounds, and no risk of interphase sample contamination.
PLG can be adapted to virtually any protocol requiring extraction of an aqueous sample with phenol and/or chloroform1. For convenience, PLG is provided predispensed into standard 2mL centrifuge tubes and comes in two varieties:
Aqueous phase: High density sample (for example, high salt content).
Organic phase: Standard mixtures of phenol, chloroform, isoamyl alcohol with a chloroform fraction of at least 60% (Phenol as the sole organic solvent is not compatible with PLG Heavy).