Integrated enzymatic fragmentation and library prep with unrivaled speed and performance
Features & Benefits
High quality libraries in 2.5 hours from 1 ng – 1 μg of input DNA
Tunable and reproducible fragmentation size range
Simple, convenient 2-step workflow with minimal hands-on time
Novel chemistry and high-fidelity amplification minimizing bias
Superior sequence coverage uniformity and low duplication rate
sparQ DNA Frag & Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
The sparQ DNA Frag & Library Prep Kit is optimized for enzymatic fragmentation of DNA and streamlined construction of high quality libraries for sequencing on Illumina NGS® platforms. The simple, convenient 2-step workflow can be completed in 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 1 ng to 1000 ng. The DNA fragmentation and polishing reactions are combined in a single step to generate 5'-phosphorylated and 3'-dA-tailed fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-Free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.