High-fidelity, high-efficiency library amplification while maintaining even coverage
Features & Benefits
HiFi DNA polymerase engineered to minimize amplification bias
Increased amplification efficiency resulting in higher yields
Uniform coverage across challenging AT- and GC-rich regions
Robust amplification from input DNA as low as 250 pg
Cost-effective alternative to KAPA HiFi with improved performance
sparQ HiFi PCR Master Mix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
sparQ HiFi PCR Master Mix is a high-fidelity, high-efficiency PCR master mix for NGS workflows requiring DNA library amplification prior to sequencing. The included primer mix allows amplification of DNA libraries flanked by adapters containing the P5 and P7 sequence elements required for Illumina® sequencing platforms. The hot-start, proofreading DNA polymerase used in the sparQ HiFi PCR Mater Mix is specifically engineered to improve library amplification efficiency while reducing PCR-derived artifacts, resulting in higher library yields and better coverage uniformity. This kit supports low DNA input from 250 pg and efficient amplification of AT- and GC rich regions with minimal bias.