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Dye reagent kit AccuMelt HRM
for genotypinggenetic

dye reagent kit
dye reagent kit
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Characteristics

Type
dye
Applications
for genotyping
Tested parameter
genetic

Description

AccuMelt HRM SuperMix maximizes differences in melt temperature and curve shape to allow discrimination of DNA sequence differences among different samples. Features & Benefits See subtle differences in sequence clearly – robust amplification ensures sufficient yield of products to generate discrete melt curves Accurate genotype calling – comparable or better performance than TaqMan® Genotyping Work with rare or precious samples – large range of template inputs possible Specificity – works with lower Mg2+ concentration than other systems thus enhancing assay accuracy AccuMelt HRM SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. Description AccuMelt HRM SuperMix is a 2x concentrated, ready to use reaction cocktail for detection of genetic variations using high resolution melting (HRM) analysis. It includes all required components except for primers and DNA template. HRM is a closed tube, rapid and cost effective procedure for characterization of sequences differences immediately following PCR amplification. It is based on the melting (dissociation) behavior of a PCR product as it transitions from double stranded to single stranded DNA in the presence of a fluorescent dsDNA binding dye.

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