AccuMelt HRM SuperMix maximizes differences in melt temperature and curve shape to allow discrimination of DNA sequence differences among different samples.
Features & Benefits
See subtle differences in sequence clearly – robust amplification ensures sufficient yield of products to generate discrete melt curves
Accurate genotype calling – comparable or better performance than TaqMan® Genotyping
Work with rare or precious samples – large range of template inputs possible
Specificity – works with lower Mg2+ concentration than other systems thus enhancing assay accuracy
AccuMelt HRM SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
AccuMelt HRM SuperMix is a 2x concentrated, ready to use reaction cocktail for detection of genetic variations using high resolution melting (HRM) analysis. It includes all required components except for primers and DNA template. HRM is a closed tube, rapid and cost effective procedure for characterization of sequences differences immediately following PCR amplification. It is based on the melting (dissociation) behavior of a PCR product as it transitions from double stranded to single stranded DNA in the presence of a fluorescent dsDNA binding dye.