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Animal research organ perfusion system

animal research organ perfusion system
animal research organ perfusion system
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for animal research
Other characteristics
isolated, tubular


The Radnoti isolated blood vessel perfusion system is designed to permit the researcher to measure the contractile responses of blood vessel segments in a temperature controlled environment. Blood vessels, or other tubular muscular structures such as small sections of intestine, are mounted on a pair of glass cannula that pass through the cap of the bath. There is one straight cannula and one J-shaped cannula per set; the vessel is attached over the end of the J-shape and connected to the straight cannula, thus forming a loop through which the vessel can be perfused. By perfusing the vessel at a set rate, changes in vessel diameter can be detected by pressure changes registered by in-line pressure transducers. The mounting cannula are set in position to accommodate the vessel’s length by positioning the cannula using the screw locks built into the cap of the bath, inserting the ends of the vessel over the end of each cannula and tying the vessel onto position. The cap also has platinum field electrodes positioned on either side of the vessel, permitting electrical stimulation of the muscle. The cap with attached vessel is then inserted into the temperature controlled bath. The bath has access ports that permit exchange of the bath fluid, either continuously or as discrete washes. Therefore, the vessel can be perfused internally and externally with similar or different fluids (even at different temperatures) and the fluids can be collected on a continual basis, if desired. The fluids can also be gassed independently, allowing the researcher to create different internal and external aerobic environments
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