Veterinary rapid test E30021 series

for swinefor antibodiesvirus

Porcine reproductive and respiratory syndrome virus (PRRSV) is a virus that causes a disease of pigs, called porcine reproductive and respiratory syndrome (PRRS), also known as blue-ear pig disease. This economically important, panzootic disease causes reproductive failure in breeding stock and respiratory tract illness in young pigs. The disease costs the United States swine industry around $644 million annually, and recent estimates in Europe found that it costs almost 1.5 billion euro every year. This kit utilizes recombinant nucleocapsid protein of PRRSV(protein N), based on which indirect ELISA or lateral flow immunoassay were further developed for laboratory testing purpose. 2. Description of Test The current PRRS Ab ELISA kit is designed to detect PRRSV antibody in swine serum samples. The 96well microtiter plate was precoated with recombinant nucleocapsid protein (protein N). During testing, samples are added into the microplate wells, in which the precoated antigen will capture the PRRSV antibody in sample and formed antigen-antibody complex. None specific antibody are discarded by a washing step. Then goat-anti-swine IgG conjugated with horseradish peroxidase (HRP) is added into each well, and further forms antibody-antigen-antibody complexes. After another washing step to remove unreacted conjugate, substrate is added and a blue color will be developed if PRRSV antibody is present. The enzyme reaction is stopped and the OD450nm value is measured. The measured intensity is positively proportional to the amount of antibody present in the sample. This ELISA kit can be used to detect PRRS antibody in swine serum.