Imaging system Intra-Vital

So far, intravital microscopy in the bone marrow is limited to a time frame of a few hours and an imaging depth of 100 -150 μm. Since immune reactions and bone healing processes take days to weeks and highly resolved, functional analysis of the dynamics in skeletal and immune system had not been achieved, we have developed a reproducible longitudinal microendoscopy technique based on gradient index (GRIN) rod lenses. The newly developed imaging technique, LIMB, enables for the first time longitudinal, dynamic in vivo studies of the osteo-immune system in the murine bone marrow. IMPLANTS MouseFix IVM Plate combined MouseFix IVM Referenze Plate thread combined MouseFix IVM endosope tube (excl. GRIN Needle Microendoscope) MouseFix screw, L=2.00 mm INSTRUMENTS IVM table & specimen holder This article needs to be customized to the laser scanning microscope CONSUMABLES Drill bit 0.30 mm Drill bit 0.31 mm Square box wrench 0.50 mm