Mycoplasma detection reagent MB000-1591

monoclonal antibodyenzymeantibiotics

Mycoplasma: Also known as mycoplasma, the smallest and simplest prokaryotic organism ever discovered, it is estimated that 15% to 35% of cells are contaminated with mycoplasma. Mycoplasma contamination can have many effects on cells, including metabolic, immune or biochemical properties, growth status, and cell survival, affecting the stability, reliability, and accuracy of experimental results; mycoplasma is difficult to detect, and It is difficult to eliminate, it can easily pass the 0.22μm standard filter, and it can also antagonize most antibiotics, causing huge losses to cell culture. Therefore, cells need to be tested for mycoplasma contamination on a regular basis (eg every 2 to 3 months). A number of methods are available for detecting mycoplasma, including broth or agar culture, direct or indirect fluorescent staining, ELISA, direct or indirect PCR, etc., wherein direct PCR is highly sensitive. To ensure the accuracy of PCR results, to prevent non-specific PCR amplification and contamination, common measures are to use hot-start Taq enzyme and UDG (uracil-DNA glycosylase). The chemically modified Taq enzyme active center is blocked such that the enzyme is not active at low temperature or normal temperature, so that non-specific amplification due to mismatching of primers is not generated prior to loading to PCR amplification. At high temperatures (such as 95 ° C), the chemically modified groups will hydrolyze off and release all enzyme activities, thus achieving the hot start of the Taq enzyme. Minimize non-specific amplification and primer dimer formation, and increase amplification sensitivity and specificity.