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UHPLC chromatography column Shim-pack MAqC-ODS I

UHPLC chromatography column
UHPLC chromatography column
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Shim-pack MAqC-ODS I reversed-phase columns are packed with a silica gel containing metal and added octadecylsilyl group. In addition to the hydrophobic characteristics of the ODS, the metal content also provides cation-exchange effects. This increases the retention of basic compounds. Therefore, this allows use with only a buffer solution as the mobile phase for analyses that previously required using an ion pair reagent and enables using gradient elution. These characteristics are especially beneficial for analyzing water soluble vitamins and pharmaceuticals that contain a large amount of basic compounds. Example of Simultaneous Analysis of Water Soluble Vitamins Water soluble vitamins contain many highly polar basic components, which are known to exhibit weak retention in the reverse-phase mode. Consequently, with typical ODS columns, such as the Shim-pack VP-ODS, an ion pair reagent is added to the mobile phase for analysis. However, using an ion pair reagent makes gradient elution difficult, resulting in peak broadening for components that take longer to elute and making it difficult to improve sensitivity. In addition, the effort required to prepare the mobile phases and condition the column is also an issue. However, because the Shim-pack MAqC-ODS I enables using gradient elution, it can shorten analysis times and result in sharp peaks even for components that elute slowly. For example, riboflavin, which elutes as the final peak with a typical ODS column, is detected with approx. 2.3 times higher sensitivity by the Shim-pack MAqC-ODS I.


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