Advances in column technology have led to a renewed interest in supercritical fluid chromatography (SFC), which uses a supercritical fluid as mobile phase. Compared to liquid, supercritical fluids have low viscosity and high diffusivity. By combining SFC with Supercritical fluid extraction (SFE), which utilizes the high permeability and diffusivity of supercritical fluids, the extraction and analysis of target components from solid samples can be automated. The new analytical technology “Unified Chromatography” improves the analytical workflow by integrating sample extraction, analysis by various separation modes, and high-sensitivity detection.
The analysis time can be effectively shortened because an excellent chromatographic resolution can be maintained even at higher flow rates than those typically used for HPLC.
Available for both UHPLC and SFC analysis using a single system : Nexera UC/s
Separation results can be quickly obtained during method development by screening with both SFC and UHPLC.
Small volume fractionation
By using a fraction collector, small-volume fractionation is also possible (ex:1.5 mL vials).
Fully automated on-line extraction and separation
Target compounds are extracted from solid samples, and then automatically transferred to SFC.
Nexera UC reduces environmental impact
By using SFC, the consumption of organic solvent is reduced by 94.2%, compared to the normal-phase.
Ideal for simultaneous analysis of compounds with a wide range of polarities
SFC can achieve a wide variety of separation patterns. This allows comprehensive analysis of compounds over a wide polarity range not previously possible with HPLC.