Protein sequencer PPSQ-51A

laboratorydata analysis

Edman degradation is used to successively cleave one amino acid (PTH-amino acid) at a time from the N-terminus of a protein, each of which is then analyzed by HPLC to determine the amino acid sequence. This diagram illustrates the process from Edman degradation to HPLC analysis of PTH-amino acids. This process is referred to as one cycle. By repeating this cycle and arranging the PTH–amino acids in the order they are obtained, the amino acid sequence of proteins and peptides can be easily and reliably determined. This process can also determine protein amino acid sequences not registered in a database. Therefore, in addition to basic research, the system can be used for a variety of other applications, such as analyzing proteins and peptides in foods or antibody therapeutics. Analysis System Lineup for Various Applications Two elution methods, isocratic and gradient, can be selected. Features Easy and Reliable Analytical System Optimized Analytical Conditions With reaction programs for Edman degradation and analytical conditions for HPLC pre-optimized, there is no need to optimize the conditions. The system’s pump delivers consistent solvent delivery, which results in chromatograms with high baseline reproducibility. In addition, the system’s high reproducibility enables subtracted chromatogram processing. This function displays the difference from the chromatogram obtained in the previous analysis, allowing uniquely increased PTH-amino acids to be easily identified. Uniquely increased PTH-amino acids to be easily identified. Simple and Easy-to-Use Data Analysis Software