Research test kit ACCEL-AMPLICON® PLUS EGFR

All-in-One Amplicon Solution The Accel-Amplicon™ EGFR Pathway Panel offers contiguous coverage of EGFR and hotspot coverage of BRAF, KRAS, and NRAS, using a 17-amplicon design to generate multiplex libraries compatible with Illumina sequencing platforms. The Accel-Amplicon EGFR Pathway Panel is compatible with short DNA fragments from both FFPE and cfDNA samples. This is especially well-suited for research focused on detecting clinically-relevant allele variants in DNA from circulating tumor cells (CTCs). This product is a complete kit that includes all elements necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters. Features: Single-tube assay Ready-to-sequence libraries in 2 hours Inputs as low as 10 ng Benefits: Average amplicon size of 136 bp for compatibility with cfDNA and FFPE Limit of detection as low as 1% On-target specificity and coverage uniformity > 95% Leverages the high fidelity performance of the Illumina platform Complete library generation in a single kit The Accel-Amplicon EGFR Pathway panel contains 17 amplicons with an average size of 136 bp that cover hotspots in four key genes in the EGFR pathway : BRAF, KRAS , NRAS , and contiguous regions of EGFR EGFR Pathway Panel Performance with Horizon’s 1% Multiplex I cfDNA Reference Standard The Accel-Amplicon EGFR Pathway Panel consistently detected validated variants at the expected frequency in three replicates from 10 ng of the Horizon Diagnostics Multiplex cfDNA Reference Product Specifications Input DNA Required 10-25 ng* Time Required 2 hours Number of Amplicons 17 Amplicon Size 107-155 bp (average 136 bp)