Research test kit Accel-NGS® Methyl-Seq

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The Accel-NGS Methyl-Seq DNA Library Kit maximizes DNA recovery of bisulfite-converted samples, to support inputs down to single cell. The kit provides the most comprehensive, uniform coverage of the methylome to support whole genome and targeted sequencing. The Accel-NGS Methyl-Seq Kit is also compatible with bisulfite-converted DNA samples enriched by ChIP or other methods as well as ancient DNA samples that may be deaminated. Features: Best sample representation Low bias library preparation Simple, 2-hour protocol Inputs from 100 pg to 100 ng Benefits: Uniform coverage improves sensitivity Reveal the complete methylome Enables low input liquid biopsy/cfDNA Compatible with multiple sample types The Accel-NGS Methyl-Seq DNA Library Kit maximizes DNA recovery of bisulfite-converted samples and constructs libraries that accurately represent sample composition. The Accel-NGS Methyl-Seq workflow maximizes DNA recovery through a post-bisulfite library preparation, utilizing a highly efficient adapter attachment that is compatible with singlestranded, bisulfite-converted DNA. Library yields from this kit are up to 100x greater than those from methods that bisulfite convert after library construction. Additionally, the templateindependent adapter attachment chemistry of the Accel-NGS Methyl-Seq Kit provides a more complete, less biased library as observed from comprehensive methylome coverage by Whole Genome Bisulfite Sequencing (WGBS). Features • High recovery of input DNA • Low bias library preparation • Simple, 2-hour library prep • Compatable with Illumina® platforms • Minimal PCR cycles required - 4 cycles for 100 ng - 7 cycles for 10 ng