The Accel-NGS Adaptase Module constructs NGS libraries from bisulfite-converted DNA from single cells. The Adaptase Module maximizes the recovery of low concentrations of single stranded DNA and libraries consistently exhibit superior complexity with reduced composition bias to provide a more faithful representation of the methylome. Using the in-line barcoding option, cells can be mulitplexed to increase throughput and further reduce library prep costs.
The Adaptase Module can be used for many applications, such as cataloging cell populations within heterogeneous tissues, assessing normal tissue for regulation of cellular mechanisms (e.g., differentiation), gaining insight into epigenetic alterations in disease states, and exploring across species to identify evolutionary conservation of epigenomic regulation.
High mapping rate
>400 bp library insert size improves data output
Workflow uses a single random priming cycle
Greater data output per run and reduced sequencing cost
Scalable cell and library indexing options for high throughput sequencing
Easier low bias workflow