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DNA extraction reagent kit Dr. GenTLE™
for nucleic acidsliquidethanol

DNA extraction reagent kit
DNA extraction reagent kit
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Characteristics

Applications
for DNA extraction, for nucleic acids
Format
liquid
Tested parameter
ethanol
Micro-organism
for yeast

Description

Takara Bio's Dr. GenTLE (Gene Trapping by Liquid Extraction) systems are designed for high quality and downstream application-ready DNA extraction and purification. Two Dr. GenTLE systems are available: 1) Dr. GenTLE (from Yeast) High Recovery (Cat. # 9082) for efficient extraction of genomic DNA from yeast cells and 2) Dr. GenTLE Precipitation Carrier (Cat. # 9094) for high efficiency ethanol precipitation of nucleic acid solutions. Dr. GenTLE (from Yeast) High Recovery includes reagents required for DNA extraction and purification from a variety of yeasts (see below) via cell wall digestion and cell lysis followed by salting-out for DNA purification. Genomic DNA isolated with this kit can be used for many applications including DNA sequencing, Southern blot analysis, cloning, PCR, and restriction enzyme digestion. Most standard procedures using 2 x 108 yeast cells obtain 4–10 µg of genomic DNA (35–200 kb). Dr. GenTLE Precipitation Carrier allows for higher DNA/RNA recovery versus glycogen-based precipitation, even from dilute (5 ng/ml) nucleic acid samples, taking advantage of a high-efficiency ethanol precipitation step during nucleic acid purification. Takara's Dr. GenTLE (from Yeast) High Recovery system is designed to enable the extraction of highly purified DNA from yeast for use in various reactions. The protocol is suitable for simultaneously processing a large number of samples. The kit is composed of three solutions: Dr. GenTLE Solution I lyses cells immediately after being added to the sample, and a noncharged complex is formed with nucleic acids.
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.