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Buffer solution reagent kit CellAmp™ Direct
for nucleic acidsfor real-time PCRfor RT-PCR

buffer solution reagent kit
buffer solution reagent kit
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Characteristics

Type
buffer solution
Applications
for nucleic acids, for real-time PCR, for RT-PCR, sample preparation

Description

The CellAmp Direct RNA Prep Kit for RT-PCR (qPCR) is for preparation of templates used in one- and two-step real-time RT-PCR, utilizing a simple method of extraction directly from cultured animal cells in 96 well-plates or other vessels. It is possible to prepare templates for real-time PCR from cultured animal cells in approximately 10 minutes. Using this kit along with a one-step real-time RT-PCR kit (such as the One Step TB Green Ex Taq qRT-PCR kit) allows gene expression analysis to be performed in approximately two hours. Templates can be prepared directly from small quantities of animal cells, which is useful for analysis of a gene expression profile and does not influence the high sensitivity of real-time RT-PCR. Genomic DNA can be removed efficiently using this kit, making it a powerful tool for expression analysis of genes for which primers can't be designed on both ends an exon junction, or genes expressed at low levels. CellAmp Direct RNA Prep Kit RT-PCR (qPCR) is for preparation of templates used in one-step and two-step real-time RT-PCR utilizing a simple method of extraction directly from cultured cells in 96 well-plates or others. It is possible to prepare templates for real-time PCR from cultured cells in approximately 10 minutes. Use of this kit with a one-step real-time RT-PCR kit (i.e. One Step TB Green Ex Taq qRT-PCR Kit) gene expression analysis can be performed in approximately 2 hours. Templates can be prepared directly from a small quantity of cells using this kit, facilitating gene expression analysis by sensitive real-time RT-PCR. Excellent for detection of rare transcripts.
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