cancer test kit / CA 15-3 / serum / colorimetric
For the quantitative determination of the cancer antigen CA15-3 concentration in human serum.
A rabbit anti-CA15-3 antibody conjugated to HRP is in the antibody-enzyme conjugate solution. The test sample reacts simultaneously with the two antibodies and is incubated. The wells are washed and color indicator is added, followed by further incubation. Once color development is stopped, the absorbance is measured spectrophotometrically at 450 nm. The concentration of CA125 is directly proportional to the color intensity of the test sample.