prostate cancer test kit / PSA / serum / colorimetric
for prostate cancer
For the quantitative determination of the total prostate specific antigen (tPSA) concentration in human serum.
Goat anti-PSA antibody directed against PSA for solid phase immobilization is used. Monoclonal anti-PSA antibody conjugated to HRP is in the antibody-enzyme conjugate solution. The test sample reacts sequentially with the two antibodies and is then incubated. The wells are then washed and color indicator is added, followed by further incubation. Color development is stopped and absorbance is measured spectrophotometrically at 450 nm. The concentration of PSA is directly proportional to the color intensity of the test sample.