The GeneArt® Genomic Cleavage Selection Kit is a rapid and reliable tool for detecting functionality of engineered nucleases in transfected cells, as well as enriching for modified cells. When using engineered nucleases to create double-stranded breaks in genomic DNA, it is useful to know whether or not the designed nucleases are functional. A way to enrich for the edited cells is also needed, especially when low cleavage activity is suspected or difficult to transfect cell lines are used. The GeneArt® Genomic Cleavage Selection Kit contains a vector with the orange fluorescent protein (OFP) gene for a quick visual check on the functionality of the engineered nuclease. In addition, the reporter genes OFP and CD4 can be used to enrich for edited cells. The vector is co-transfected with genome editing tools such as zinc-finger nuclease (ZFN), transcriptional activator-like effector nucleases (TALENs), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) with results 24-72 hours post transfection.