Solution reagent kit RelaxGene

blood samplefor DNA extractiongenomic DNA

Extraction of genomic DNA from 0.1-20 ml fresh and cryopreserved blood of various anticoagulants Storage This kit can be stored under dry condition at room temperature (15-25℃ ) for 12 months. For longterm storage, please store at 2-8℃ . Description The RelaxGene Blood DNA System adopts a unique buffer system to extract genomic DNA from 0.1-20 ml of fresh blood with various anticoagulants and cryopreserved blood samples. The buffer system can remove the proteins, pigments, lipids and other inhibitory impurities to a maximum extent, and obtain large size DNA fragments with high yield, high purity and good stability. The kit does not need to use organic solvents such as phenol/chloroform. The purified DNA can be used in various regular experiments such as restriction digestion, PCR, library construction, Southern blot (high throughput sequencing chip hybridization), etc. Features ■ Flexible sample starting amount: 2-400 μg high purity DNA can be extracted from 0.1-20 ml of various blood samples. ■ Safe and reliable: No contamination of organic solvents such as phenol/chloroform. Applications Suitable for various regular applications, such as restriction digestion, PCR, library construction, Southern blot (chip hybridization of high-throughput sequencing), etc. Purification of the genomic DNA from 300 μl, 5 ml and 10 ml human anticoagulation blood with EDTA using RelaxGene Blood DNA System . 3 μl of the 200 μl, 500 μl and 1 ml eluates respectively were loaded per lane. M: TIANGEN Marker D15000. Experimental results: 0.1-20 ml of blood can be extracted by RelaxGene Blood DNA System with good linear range.