The AutoDBS-3 is the first in a line of next generation instruments that leverages Tomtec advancements in pre-etched PDMS cards. The AutoDBS-3 is a proof of concept platform that allows users to deploy a full GLP workflow for DBS-bio sampling leveraging the Tomtec family of pre-etched PDMS cards. This semi-manual instrument works in conjunction with a MS Excel driven application running on a connected Windows based personal computer:
1) The PDMS card is placed on the instrument and bar-code scanned.
2) Motors then place the highlighted spot over the target assay well.
3) The user is then prompted to punch the highlighted spot into the assay.
4) The user clicks Next on the PC, and the procedure repeats. This is done for all nine spots on the card.
After the card is punched, it is removed from the instrument and the process starts again. Although this is not a walk-away automation workflow, the platform does provide full GLP and Chain of Custody for the transfer process. Follow-up generations will leverage the automation techniques Tomtec has developed over decades and apply that knowledge to fully automating the harvest and transfer of PDMS targets to 96-well assays.