Research chromatography column Proteus NoEndo17E

for protein purificationhigh-capacity

Overview
Proteus NoEndo-17-E spin columns provide fast, affinity-based removal of endotoxins from antibodies and other proteins. The FlowGo-17-E design combines gravity-column quality separations with centrifugal speed. Typical single-pass endotoxin removal reaches about 99% with protein recoveries ≥85%. Formats include Micro, Mini, Standard (pre-packed) and High Capacity (HC); HC columns are recommended for recombinant proteins from bacteria (e.g. E. coli) and Standard columns for monoclonal antibodies from mammalian cells. Mini and Micro kits include loose resin for sample volumes ≤20 ml.

Performance Characteristics

• Formats: Micro | Mini | Standard | High Capacity
• Typical in situ binding capacity: 300–500 EU | 3,000 EU | 30,000 EU | 500,000–1,000,000 EU
• Typical binding capacity per ml resin: 500–800 EU/ml | 300 EU/ml | 1,500 EU/ml | 30,000 EU/ml
• Minimum endotoxin levels tested post-column: <0.03 EU/ml | <0.03 EU/ml | <0.05 EU/ml | <0.05 EU/ml
• Typical endotoxin clearance after 1 pass: (Micro/Mini not listed) | (Micro/Mini not listed) | 3 log reduction | 3 log reduction
• Typical endotoxin clearance after 2 passes: (Micro/Mini not listed) | (Micro/Mini not listed) | 4 log reduction | 4 log reduction
• Typical endotoxin clearance after 1 hour incubation: 3 log reduction | 2 log reduction | (not listed) | (not listed)
• Typical endotoxin clearance after 3 hour incubation: 4 log reduction | 3 log reduction | (not listed) | (not listed)
• Maximum sample load volume: 0.6 ml | 20 ml | 20 ml | 20 ml
• Bed volume: 0.01–0.1 ml loose | 0.25 ml loose | 1 ml pre-packed | 1.7 ml pre-packed
• Bead size: ~100 µm (all formats)
• Recommended working pH: 4–8 (all formats)
• Color-coded end-caps: supplied in vials (Micro/Mini) | light green (Standard) | dark green (High Capacity)

Technical Specifications

• Applies to: NoEndo-17-E S, HC & M and NoEndo-17-E Micro
• Sample capacity (by rotor type): Swinging bucket: 20 ml (S/HC/M), NOT USABLE (Micro). Fixed angle: 12 ml (250) (S/HC/M), 0.6 ml (Micro).
• Dimensions: Length: 118 mm (S/HC/M) | 48 mm (Micro). Width: 29 mm | 11 mm. Hold-up volume: 10 µl | 2–3 µl.
• Materials of construction: Outer tube: polypropylene (all). Inner tube: polyethylene (S/HC/M) | polypropylene (Micro). Resin sintered plug: polyethylene (S/HC/M) | N/A (Micro). NoEndo resin: cross-linked 6% agarose (all).
• Centrifuge required: Rotor cavity to fit 50 ml conical bottom tubes (S/HC/M) or to fit 2.2 ml centrifuge tubes (Micro). Maximum speed: 1,500 x g (fixed angle or swinging bucket for S/HC/M); 13,000 x g (fixed angle ONLY for Micro).

Ordering (available packs & prices)

• GEN-NoE2MICRO — 2 spin columns, 4 centrifuge tubes, 1 vial of resin (0.2 ml) — $150.00
• GEN-NoE2M — 2 spin columns, 2 centrifuge tubes, 2 vials of resin (0.25 ml each), 2 incubation caps — $210.00
• GEN-NoE2S — 2 spin columns, 2 centrifuge tubes, 2 standard resin cartridges, 1 insertion tool — $220.00
• GEN-NoE2HC — 2 spin columns, 2 centrifuge tubes, 2 high capacity resin cartridges, 1 insertion tool — $290.00
• GEN-NoE12S — 12 spin columns, 12 centrifuge tubes, 12 standard resin cartridges, 1 insertion tool — $960.00
• GEN-NoE12HC — 12 spin columns, 12 centrifuge tubes, 12 high capacity resin cartridges, 1 insertion tool — $1,180.00
• GEN-NoE12M — 12 spin columns, 12 centrifuge tubes, 12 vials of resin (0.25 ml each), 12 incubation caps — $850.00
• GEN-NoE24MICRO — 24 spin columns, 48 centrifuge tubes, 1 vial of resin (2.4 ml) — $680.00
• GEN-NoE48S — 48 spin columns, 48 standard resin cartridges, 1 insertion tool (no centrifuge tubes) — $3,000.00
• GEN-NoE48HC — 48 spin columns, 48 high capacity resin cartridges, 1 insertion tool (no centrifuge tubes) — $3,420.00
• GEN-NoE100ML — 100 ml NoEndo loose resin suspended in 20% ethanol — $3,000.00

Features / Technical details

• Resin: cross-linked 6% agarose NoEndo resin.
• Typical protein recovery: ≥85% (single pass when used as directed).
• Endotoxin removal: up to ~99% in a single pass; typical 3 log reduction (Standard, HC) after 1 pass; up to 4 log reduction after 2 passes (Standard, HC).
• Minimum endotoxin levels post-column: as low as <0.03–0.05 EU/ml depending on format.
• Formats: Micro, Mini, Standard (pre-packed), High Capacity (pre-packed), and Loose Resin (kits and bulk 100 ml).
• Maximum sample load volumes: Micro 0.6 ml; Mini/Standard/HC up to 20 ml (depending on rotor type).
• Bead size: ~100 µm.
• Recommended working pH: 4–8.
• Materials: polypropylene outer tube; polyethylene or polypropylene inner components depending on format.
• Centrifuge: specific rotor cavity/adapter requirements; Micro format supports high speed (up to 13,000 x g fixed-angle), S/HC/M formats up to 1,500 x g.