Hepatitis B test kit HBsAg ELISA
for antigensHBVELISA

hepatitis B test kit
hepatitis B test kit
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Characteristics

Applications
hepatitis B
Tested parameter
for antigens
Micro-organism
HBV
Analysis mode
ELISA

Description

PRINCIPLE The sample is placed in contact with an anti-HBs monoclonal antibody immobilized onto a solid support. If it contains the HBsAg antigen, it will form a complex with the antibodies and will bind to the support. The unbound fraction is eliminated by washing, after which another anti-HBs monoclonal antibody conjugated to peroxidase is added. If a reaction was produced during the first stage of the process, the conjugate will bind. After a new wash the enzymatic substrate is added. If HBsAg is present in the sample, a light blue color is developed. The reaction is stopped by adding sulfuric acid, which makes the light blue color change to yellow. PROVIDED REAGENTS Coated microtitration plate: microtitration plates with removable strips with wells containing immobilized anti-HBs monoclonal antibody. Concentrated Conjugate: anti-HBs monoclonal antibody conjugated to peroxidase. Conjugate Diluent: Tris buffer containing additives and preservatives. Substrate A: 60 mmol/l hydrogen peroxide in 50 mmol/l citrate buffer. Substrate B: 0.01 mol/l tetramethylbenzidine (TMB) in 0.1 N hydrochloric acid. Stopper: 2 N sulfuric acid. Concentrated Wash Buffer: 1.4 mol/l sodium chloride in 100 mmol/l phosphate buffer and 0.1 g/l non-ionic surfactant. Positive Control: dilution of inactivated serum reactive to HBsAg. Negative Control: dilution of non-reactive inactivated serum.

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