Chagas disease test kit lisado
ELISA

Chagas disease test kit
Chagas disease test kit
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Characteristics

Applications
for Chagas disease
Analysis mode
ELISA

Description

PRINCIPLE Chagatest ELISA lisado is an "in vitro" enzyme immunoassay for the qualitative detection of antibodies anti-T. cruzi in human serum or plasma samples. The sample is diluted in the wells in which T.cruzi antigens are immobilized, corresponding to highly preserved zones among different strains. If the sample contains specific antibodies, they will bind to the antigens and remain bound to the solid phase. Unbound fractions are eliminated by washing, after which conjugate is added (monoclonal antibodies anti-human IgG, conjugated to peroxidase), which specifically reacts with immunocaptured antibodies anti-T.cruzi. The unbound conjugate is removed by washing. The presence of peroxidase bound to the complex is recognized by the addition of a chromogenic substrate, tetramethylbenzidine. The reactive samples develop a light blue color. The enzymatic reaction is stopped by the addition of sulphuric acid, producing a light blue to yellow color change. The optical density is measured bichromatically at 450/620-650 nm or at 450 nm. PROVIDED REAGENTS Coated microtitration plate: microtitration plate with removable strips and 96 wells containing Trypanosoma cruzi antigens. Sample Diluent: saline buffer with surfactant. Violet color. Concentrated Conjugate: monoclonal antibody anti-Human IgG, conjugated to peroxidase (10x). Red color. Conjugate Diluent: saline buffer with proteins. Substrate: tetramethylbenzidine and hydrogen peroxide solution. Stopper: 2 N sulfuric acid. Concentrated Wash Buffer: saline buffer with surfactant (25x). Green color.

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