The microtitration plate wells are coated with HIV-1 and HIV2 recombinant proteins and synthetic peptides and anti-p24
monoclonal antibodies(*). The sample is incubated in the
wells, if it contains p24 and/or HIV-1 (gp41) or HIV-2 (gp36)
antibodies they will bind to the antigens and/or antibodies
present in the wells. The unbound fraction is eliminated
by washing. The next step is the addition of Conjugate 1
containing antibodies and antigens marked with biotin that
will bind to antigens/antibodies is they are present in the
sample. Then Conjugate 2 is added (peroxidase conjugated
to streptavidin) that will bind to Conjugate 1. The unbound
conjugate is removed by washing.
Then, a solution containing tetramethylbenzidine (TMB) and
hydrogen peroxide is added. The reactive samples develop
a blue color that changes to yellow when the reaction is
stopped with sulfuric acid (Stopper).
Coated microtitration plate: 96 wells microtitration plate
coated with HIV-1 and HIV-2 recombinant proteins and synthetic peptides and human anti-p24 monoclonal antibodies.
Sample Diluent: 0.02 M Tris buffer containing bovine proteins, sodium chloride and surfactant agent, pH 7.2, blue