AIDS test kit 1723451
for antigensfor antibodiespH

AIDS test kit
AIDS test kit
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Characteristics

Applications
AIDS
Tested parameter
for antigens, for antibodies, pH, hydrogen peroxide, chloride
Micro-organism
HIV
Sample type
serum, plasma
Analysis mode
ELISA
Specificity

99.9 %

Description

PRINCIPLE The microtitration plate wells are coated with HIV-1 and HIV2 recombinant proteins and synthetic peptides and anti-p24 monoclonal antibodies(*). The sample is incubated in the wells, if it contains p24 and/or HIV-1 (gp41) or HIV-2 (gp36) antibodies they will bind to the antigens and/or antibodies present in the wells. The unbound fraction is eliminated by washing. The next step is the addition of Conjugate 1 containing antibodies and antigens marked with biotin that will bind to antigens/antibodies is they are present in the sample. Then Conjugate 2 is added (peroxidase conjugated to streptavidin) that will bind to Conjugate 1. The unbound conjugate is removed by washing. Then, a solution containing tetramethylbenzidine (TMB) and hydrogen peroxide is added. The reactive samples develop a blue color that changes to yellow when the reaction is stopped with sulfuric acid (Stopper). PROVIDED REAGENTS Coated microtitration plate: 96 wells microtitration plate coated with HIV-1 and HIV-2 recombinant proteins and synthetic peptides and human anti-p24 monoclonal antibodies. Sample Diluent: 0.02 M Tris buffer containing bovine proteins, sodium chloride and surfactant agent, pH 7.2, blue color.

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*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.