With structured illumination, removal of out-of-focus light becomes simple and efficient. ZEISS Apotome 3 calculates your optical section from a number of images acquired with different grid positions. Get high-contrast images, even from thicker specimens, while your system remains just as easy to operate as always.
Brilliant optical sections
Based on peer-reviewed algorithms
More structural information
Free choice of light source and dyes
Brilliant Optical Sections
Even From Thick Specimens
Apotome 3 significantly increases the axial resolution compared to conventional fluorescence microscopy: You obtain optical sections that allow 3D rendering, even from thick specimens. Three grids of different geometries give you the best resolution for each objective. You can focus on your experiment as the ideal grid is automatically selected, always resulting in high-contrast optical sections.
Peer-reviewed Algorithms
Linear Approaches for True Optical Sections
Software-based solutions require either prior knowledge of the sample (AI based methods) or rely on complex algorithms that have not been peer-reviewed. Users must trust that these black-box solutions only produce structures that are real. ZEISS Apotome 3 uses linear approaches and well documented algorithms, allowing you to calculate true, reliable optical sections.
More Structural Information
Compare Widefield, Optical Sectioning, and Deconvolution
Improve your images even more by deconvolution, using a patented algorithm for structured illumination. While retaining all raw data, the system allows you to switch between widefield, optical section