rapid AIDS test / HIV / for DNA / ELISA
D5325 / D5326

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rapid AIDS test / HIV / for DNA / ELISA rapid AIDS test / HIV / for DNA / ELISA - D5325 / D5326
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Characteristics

  • Applications:

    AIDS

  • Tested parameter:

    HIV

  • Sample type:

    for DNA

  • Analysis mode:

    ELISA

Description

HIGHLIGHTS
For high-throughput detection of global 5-methylcytosine (5-mC) in DNA
The streamlined workflow can be completed in less than 3 hours

DESCRIPTION
The 5-mC DNA ELISA Kit allows researchers to accurately quantitate 5-mC in any DNA sample in less than 3 hours. The kit features an Anti-5-Methylcytosine monoclonal antibody that is both sensitive and specific for 5-mC. The assay is compatible with a wide range of input DNA from vertebrate, plant, and microbial sources as well as methylated PCR amplicons and fragmented DNA. Percent 5-mC in a DNA sample can be accurately quantified from a standard curve generated with specially designed controls included with the kit. Also, the fast, streamlined workflow is ideal for high-throughput analyses.

TECHNICAL SPECIFICATIONS
Detection> 0.5% 5-mC per 100 ng DNA

EquipmentIncubator and ELISA plate reader. A multi-channel pipettor is recommended. An automated plate washer may be used for blocking and wash steps.

InputThis protocol is optimized for 100 ng input DNA/well. Compatible with DNA in the range of 10-200 ng/well.

Sample SourcePurified genomic DNA, plasmid DNA, PCR amplification products, or DNA fragments in water, Tris-EDTA, or similar.