Antibody RM17575
diagnosticfor ELISARNA Synthesis

antibody
antibody
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Characteristics

Type
antibody
Applications
diagnostic, for ELISA, RNA Synthesis
Tested parameter
protein
Micro-organism
SARS-COV-2, coronavirus, MERS-CoV
Origin
animal-based
Storage temperature

Min.: -80 °C
(-112 °F)

Max.: 2 °C
(36 °F)

Description

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. Immunogen - E.coli derived SARS-COV-2 Nucleoproteins Ser2-Ala419(Gly335Ala) Accession #QHD43423.2 Source - Rabbit Purirty - Affinity purification Ig Type - Rabbit IgG Endotoxin Level - < 1.0 EU/μg of the protein by L AL method. Formulation - Supplied as a 0.2um filtered solution in PBS with 0.1%Braveds MB-1, PH 7.4. CrossReactivity - Has cross-reactivity in ELISA with Recombinant SARS-COV-2 Nucleocapsid Protein with His tag No cross-reactivity in ELISA with MERS-CoV Nucleoprotein protein HCoV-229E Nucleoprotein protein Avoid repeated freeze-thaw cycles.

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