Enzyme reagent A20022

immunohistochemistryfor immunofluorescenceprotein

Host species - Rabbit Purification method - Affinity purification Isotype - IgG Background The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. Applications Recommended dilution - ELISA 1:1000 - 1:5000 WB 1:2000 - 1:10000 FC 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 1:50 - 1:200 Storage buffer - Store at 4℃ Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, pH7.3. Application key - Western blotting Flow Cytometry Immunofluorescence Immunoprecipitation Positive samples - 293T