Enzyme reagent RK21101

for microbiologypHglycerol

Unit Definition - One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA fragments in 1 hour at 37°C in a total reaction volume of 50 µl. Storage Conditions - 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml BSA, 50% Glycerol, pH 7.4 @ 25°C Reaction Conditions - 1X Buffer CutC, Incubate at 37°C. Reaction Buffer or Mix - 1X CutC Buffer: 100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 100 μg/ml BSA, pH 7.9 @ 25°C Heat Inactivation - No Methylation Sensitivity - dam methylation - Not Sensitive dcm methylation - Not Sensitive CpG Methylation - Not Sensitive